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Semen Analysis (Sperm Test)

A semen analysis is a laboratory test of a semen sample that measures the volume, sperm count (concentration), motility (movement) and morphology (shape) of sperm, assessed against WHO reference values. It is the first and most important test for male fertility, and is also used before sperm freezing or to confirm a vasectomy. At Aansh Hospital & IVF Center — a government-registered Level-2 ART clinic (Reg. No. MH/AC/2024/15441/L2/Chandrapur/132) — the test is performed in our in-house andrology lab, confidentially, usually with a same-day report. Confidential and straightforward. A semen analysis is a simple, private test. The sample is processed in our in-house andrology lab by Senior Clinical Embryologist Aayush Agarwal, Ph.D., and results are reviewed with the couple by Dr. Shweta Agarwal (MBBS, DGO). You can ask questions discreetly on WhatsApp before booking. In Marathi and Hindi, this test is known as वीर्य विश्लेषण (semen analysis / वीर्य चाचणी). Looking for what your numbers mean? This page explains the test itself — preparation, collection and cost. For a plain-language guide to interpreting your report (count, motility, morphology and the WHO reference values), read Semen analysis: what your numbers mean.

Medically reviewed by Dr. Shweta Agarwal, MBBS, DGO · Last updated June 2026
Dr. Shweta Agarwal, Founder & Lead Fertility Specialist, at Aansh Hospital & IVF Center, Chandrapur Govt. ART-registered
Dr. Shweta Agarwal MBBS, DGO · Reproductive Medicine
5,000+IVF babies
30+Years of experience
4.9★500+ reviews · Google, JustDial, Practo
94%AI embryo-analysis accuracy · Garbha.ai
ART Level 2 RegisteredGovt. of India — ART Act 2021
Dr. Shweta AgarwalMBBS, DGO · Reproductive Medicine
On-site embryology labLed by Aayush Agarwal, Ph.D.
Marathi · Hindi · EnglishChandrapur · Nagpur · Vidarbha

Medically reviewed by Dr. Shweta Agarwal, MBBS, DGO. Last updated: June 2026.

Information on this page is educational and does not replace a medical consultation. Outcomes depend on individual clinical factors.


How should I prepare for a semen analysis?

Prepare with 2 to 5 days of sexual abstinence before the test — this window gives the most reliable, representative result. Ejaculating less than 2 days before can lower the measured count, while waiting more than about 7 days can reduce motility.

In the days before the test:

  • Abstain for 2–5 days (no more, no less, for the most accurate result).
  • Avoid alcohol and smoking in the week prior where possible.
  • Avoid starting new medications; tell your doctor about any you take regularly.
  • Stay hydrated and well-rested, and avoid a high fever in the preceding weeks (fever can temporarily lower sperm quality).

If you have had a fever or illness recently, mention it — your doctor may suggest timing the test accordingly.


How is the sample collected? (Privacy)

The sample is collected by masturbation into a sterile container provided by the clinic. A private, comfortable room is available at the centre for collection, so you do not need to travel with a sample. The process is confidential and handled with discretion.

If you prefer to collect at home, the sample must reach the lab within 30–60 minutes, kept close to body temperature (not refrigerated) and protected from cold, as delays and temperature changes affect the result. Lubricants and ordinary condoms should not be used, as they can damage sperm; the lab can advise on a suitable collection method.

Your privacy is protected throughout — collection, handling and reporting are kept confidential.


What parameters does a semen analysis measure?

A semen analysis assesses several parameters together to build a picture of sperm quality, evaluated against WHO reference values:

Parameter What it measures
Volume The amount of semen in the sample
Sperm concentration (count) Number of sperm per millilitre
Total & progressive motility The percentage of sperm moving, and moving forward effectively
Morphology The percentage of normally shaped sperm (strict criteria)
pH Acidity/alkalinity of the semen
Liquefaction time How quickly the sample liquefies
White blood cells A marker of possible infection or inflammation

Together these indicate whether a male factor may be contributing to difficulty conceiving. A single test is not a diagnosis — see below.

For a detailed, plain-language explanation of each number and what counts as a normal range, read our guide: Semen analysis: what your numbers mean.


When is a semen analysis recommended?

A semen analysis is recommended in several situations — it is not only for couples facing infertility:

  • Difficulty conceiving: After 12 months of trying (or 6 months if the female partner is 35 or older). It is part of the complete couple fertility workup.
  • Before fertility treatment: To plan IUI, IVF or ICSI.
  • Before sperm freezing or fertility preservation (for example, before cancer treatment).
  • To confirm a vasectomy has worked (no sperm remaining).
  • Known risk factors: Previous testicular injury, undescended testes, infection (such as mumps), or scrotal surgery.

How long does it take, and how accurate is one test?

Sample collection and lab processing typically take 1–2 hours, and a written report is usually ready the same day. Your results are then reviewed with you, in the context of your full history, before any conclusions are drawn.

One abnormal result does not confirm infertility. Sperm parameters naturally fluctuate with illness, fever, stress, and lifestyle. When a result is abnormal, the test is usually repeated after about 4–6 weeks to confirm the finding before any treatment is recommended. If results remain abnormal, further evaluation for male infertility — hormone tests, scrotal ultrasound, or genetic tests — may follow, and treatment is matched to the cause (for example, surgical sperm retrieval with ICSI for azoospermia).


How much does a semen analysis cost?

A semen analysis is a modest, standalone diagnostic test, priced separately from any treatment. You are told the cost before booking, and a repeat or confirmatory test (if advised) is discussed transparently.

See /costs-emi for current pricing information.

If treatment is later needed, you receive a transparent written estimate, and 0% EMI (3–24 months) is available. See IVF cost & 0% EMI for treatment pricing.


Good to know

Frequently asked questions

How should I prepare for a semen analysis?
Abstain from ejaculation for 2 to 5 days before the test — this gives the most reliable result. Avoid alcohol and smoking in the week prior where possible, avoid starting new medications, and stay hydrated and well-rested. Ejaculating less than 2 days before can lower the count, while waiting more than 7 days can reduce motility.
How is the sample collected, and is it private?
The sample is collected by masturbation into a sterile container, in a private, comfortable room at the centre. The process is confidential. If you collect at home, the sample must reach the lab within 30–60 minutes at body temperature, and ordinary lubricants or condoms must be avoided as they can damage sperm.
What does a semen analysis measure?
It measures semen volume, sperm concentration (count), motility (movement), and morphology (shape), along with pH, liquefaction time and white blood cells, all assessed against WHO reference values. Together these indicate whether a male factor may be affecting fertility. For what each number means, see our guide on interpreting your report.
Can one abnormal result confirm infertility?
No. Sperm parameters fluctuate with illness, fever, stress and lifestyle, so a single abnormal result is not a diagnosis. The test is usually repeated after 4–6 weeks to confirm the finding before any treatment is recommended. Results are always reviewed alongside your complete medical history.
How long does the test take, and when do I get results?
Sample collection and lab processing usually take 1 to 2 hours, and the written report is typically ready the same day. The results are then reviewed with you in a follow-up consultation, where the findings and any recommended next steps are explained in plain language.
Is a semen analysis only for infertility?
No. It is also used to confirm a vasectomy has worked, before sperm freezing, and during fertility-preservation planning (for example, before cancer treatment). It is a versatile test of male reproductive health, used whenever sperm quality or quantity needs to be assessed.
What happens if my results are abnormal?
Treatment depends on the specific abnormality. Mild issues may respond to lifestyle changes; moderate issues may be treated with IUI; severe low counts or azoospermia may need ICSI with surgical sperm retrieval. A personalised plan is built around your exact diagnosis as part of male infertility care.
Will the test and my results stay confidential?
Yes. Collection, handling and reporting are kept private, and the sample is processed in our in-house andrology lab. You can ask questions and share concerns discreetly on WhatsApp before booking. Male fertility is discussed sensitively and as part of the couple's overall care.
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